Comments on: microRNA Target Prediction Tools http://mirnablog.com/microrna-target-prediction-tools/ miRNA Research & Industry News Thu, 02 Feb 2012 18:01:02 +0000 hourly 1 http://wordpress.org/?v= By: Amy Palmer http://mirnablog.com/microrna-target-prediction-tools/comment-page-1/#comment-925 Amy Palmer Tue, 20 Sep 2011 20:10:21 +0000 http://mirnablog.com/?p=315#comment-925 IPA's new microRNA Target Filter is a new microRNA target prioritization tool that is available within IPA. It would be great to see it added to this list. It contains content from TarBase, TargetScan, miRecords, and micro-RNA related findings from the published literature. It also includes filtering tools that help you quickly sort through thousands of microRNA targets to find the ones most biologically relevant to your dataset or experiment. See the poster that describes how this works at: http://www.ingenuity.com/library/pdf/poster_microRNA_melanoma.pdf. IPA’s new microRNA Target Filter is a new microRNA target prioritization tool that is available within IPA. It would be great to see it added to this list. It contains content from TarBase, TargetScan, miRecords, and micro-RNA related findings from the published literature. It also includes filtering tools that help you quickly sort through thousands of microRNA targets to find the ones most biologically relevant to your dataset or experiment. See the poster that describes how this works at: http://www.ingenuity.com/library/pdf/poster_microRNA_melanoma.pdf.

]]> By: mohit http://mirnablog.com/microrna-target-prediction-tools/comment-page-1/#comment-262 mohit Tue, 12 Apr 2011 12:53:50 +0000 http://mirnablog.com/?p=315#comment-262 Hi there, I am working with plants miRNAs and currently at stage of validating inhibition of targets mRNA by miRNA with help of RNA ligase mediated RACE. Although RACE manual says to use gene specific primers for cDNA preparations I would like to use oligo dT primer for cDNA preparation. I welcome all suggestions regarding use of oligo dT primer for cDNA preparations. RACE expts are planned to be done with use of gene specific primers. Also any reference regarding the same are appreciable. Hi there,
I am working with plants miRNAs and currently at stage of validating inhibition of targets mRNA by miRNA with help of RNA ligase mediated RACE. Although RACE manual says to use gene specific primers for cDNA preparations I would like to use oligo dT primer for cDNA preparation. I welcome all suggestions regarding use of oligo dT primer for cDNA preparations. RACE expts are planned to be done with use of gene specific primers. Also any reference regarding the same are appreciable.

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