The hippocampus is a part of the brain critically involved in memory formation and spatial navigation. Shinohara et al. (2011,) used deep sequencing for miRNA profiling of multiple samples of the mature male rat hippocampal CA3 region. According to their study, the let-7 family miRNA represents about half of the small RNA cDNA library. Major let-7 family miRNAs are let-7c (23%), let-7a (9%), let-7f (9%), and let-7b (7%). The authors found miR134, a miRNA previously reported to be related to synaptic plasticity and morphology, only modestly expressed (~0.05%). The miRNA profiles of the left and right hippocampi were similar. The sequence data derived from 3 pooled data sets and each data set contained corresponding left and right hippocampal CA3 samples. This study is one of the fewest studies in which miRNA expression profiles were quantitatively assessed in a specific region of the brain. The raw sequence reads and cross-mapped results are available in the DDJB sequence archive with the accession number DRA000379 (http://trace.ddbj.nig.ac.jp/DRASearch/submission?acc=DRA000379).
miRNA profiling of bilateral rat hippocampal CA3 by deep sequencing.
Shinohara Y, Yahagi K, Kawano M, Nishiyori H, Kawazu C, Suzuki N, Manabe RI, Hirase H.
Biochem Biophys Res Commun. 2011 May 13. [Epub ahead of print]
PMID: 21575607 [PubMed – as supplied by publisher]