Resveratrol (Res) has anticancer activity in prostate cancer (PCa), which can be attributed to modulation of microRNAs (miRNAs/miRs). miRNAs/miRs are small non-coding RNAs that negatively regulate gene expression. We have analyzed differential miRNA expression in PCa cells treated with Res.
Using miRNA microarrays at the University of Mississippi Medical Center found that 23 miRNAs were significantly down-regulated and 28 miRNAs were significantly up-regulated after Res treatment. The down-regulated miRs included miR-17-92 and miR-106ab clusters with well recognized oncogenic properties while the up-regulated miRs included several tumor suppressors. Selected miRs were verified by qRT-PCR, including miR-17, miR-20a, miR-20b, miR-106a and miR106b. Since these miRNAs target PTEN (phosphatase and tensin homolog deleted on chromosome 10), they performed Western blot to confirm up-regulation of PTEN in PCa cells. In addition, using TargetScan database, they have identified putative mRNA targets for Res-induced down- and up-regulated miRs. Using a bioinformatics approach, the authors generated gene networks specifically altered by Res-regulated miRNAs.
These results indicate that the dietary compound Res may play an important role in prostate carcinogenesis through modulation of miRNA expression: Res down-regulated oncogenic miRs and up-regulated tumor suppressor miRs in PCa cells. Further in-depth studies are necessary in order to fully recognize the beneficial miRNA-mediated effects of Res in PCa.
Dhar S, Hicks C, Levenson AS. (2011) Resveratrol and prostate cancer: Promising role for microRNAs. Mol Nutr Food Res [Epub ahead of print]. [abstract]