Ovarian cancer is the leading cause of death from gynecologic cancer in women worldwide. According to the National Cancer Institute, ovarian cancer has the highest mortality rate among all the reproductive cancers in women. Advanced stage diagnosis and chemo/radio-resistance is a major obstacle in treating advanced ovarian cancer. The most commonly employed chemotherapeutic drug for ovarian cancer treatment is cis-platin. As with most chemotherapeutic drugs, many patients eventually become resistant to cis-platin and therefore, diminishing its effect. The efficacy of current treatments may be improved by increasing the sensitivity of cancer cells to chemo/radiation therapies.
This study is focused on identifying the differential expression of regulatory microRNAs (miRNAs) between cis-platin sensitive (A2780), and cis-platin resistant (A2780/CP70) cell lines. Cell proliferation assays were conducted to test the sensitivity of the two cell lines to cis-platin.
Differential expression pattern analysis reveales changes in expression of 11 miRNAs out of 1,500 miRNAs analyzed. Out of the 11 miRNAs identified, 5 were up-regulated in the A2780/CP70 cell line and [click to continue…]
Heart disease and cancer represent the number one and number two killer diseases in developed countries. Identifying biomarkers to detect these and other diseases at early stages remains an important research goal and researchers are scrambling for ways to identify contributing factors to the pathogenesis of diseased cells in the body. In recent years, research has turned to the study of microRNA (miRNA) as possible biomarkers due to their extensive role in biological processes and cell functionality in normal vs. diseased cells. Microarrays combined with quantitative real-time PCR (qRT-PCR) validation are proving to be valuable tools for miRNA expression profiling and are predicted to play a crucial role in biomarker discovery and detection. [click to continue…]
This month, GT brings you a technical guide on manipulating microRNAs. They have consulted some of the best and brightest in the field to get feedback on how best to perform both discovery and expression profiling studies. Their troubleshooting advice ranges from how to isolate and purify miRNA from cells, to how to up- or down-regulate a specific miRNA and how to verify the results. (download PDF)
MicroRNAs (miRNAs) have proven to be an extremely important part of the gene expression regulation mechanism. Expression profiling and functional studies indicate that miRNAs participate in the regulation of almost every cellular process investigated and that changes in their expression have a profound effect on their gene targeting activities. While the role of miRNAs in cell development and tumorigenesis was recognized early on, the importance of these small RNAs on immune system development and function has only been touched on recently. Initial studies have indicated that miRNAs have a role in the regulation of immune response and inflammation. Although inflammation protects against pathogenic stimuli and promotes healing, it may actually cause more damage than the activating event if not strictly controlled by essential, internal negative regulators. Out of control immune response causes various forms of chronic inflammatory diseases. [click to continue…]