microrna microarray

Product innovation and the development of high-qualitative assays are the need of the hour

LONDON, Sept. 4, 2013 /PRNewswire/ — Rapid growth in microRNA (miRNA) research over the past two years, owing to its diagnostic and therapeutic potential, has fueled development in the global miRNA tools and services market. Of the various miRNA technologies, which include quantitative real-time polymerase chain reaction (qRT PCR), microarrays and functional tools, qRT PCR remains the fastest-growing, while microarray is gradually being replaced by next-generation sequencing platforms.

New analysis from Frost & Sullivan (http://www.frost.com/prod/servlet/svcg.pag/HCLS), Global Analysis of MicroRNA Tools and Services Market, finds that the tools side of the market earned revenues of $110.0 million in 2012 and estimates this to more than double to reach $247.7 million in 2017. The US contributed to about 47% of the revenue followed by 36.7% from Europe, while leaving the rest to APAC and ROW. The miRNA services market earned revenues of $35.1 million in 2012 and this is expected to grow to $63.3 million in 2017. The end users covered are academic and research institutes, core facilities, and pharmaceutical and biotech companies.

The growth of therapeutic and diagnostic enterprises, higher research funding and increased outsourcing to contract research organizations in the US will present immense opportunities for the miRNA tools and services market. At the same time, [click to continue…]

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Houston, TX (PRWEB)   -  A week after the latest miRBase update LC Sciences announced use of miRBase Version 17 probe content as new default for their miRNA microarrays. Arrays for all species included in the latest miRBase 17 release are available. Looking at the most common species: while the changes in probe content for rat are negligible, additions to the list of human miRNAs are substantial: 521 new unique human mature miRNA sequences were added (see our post miRBase Version 17 Update Released).

The full press release: http://www.prweb.com/releases/2011/5/prweb8404968.htm

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microrna microarraysby Jeffrey M. Perkel

If PubMed is any guide, the microRNA field is smokin’ hot. Of the nearly 11,100 references that come up in a search for “microRNA,” nearly two-thirds (64%) were published since 2009.

It’s no wonder. Although microRNAs (miRNAs) may be small—they average 22 nucleotides in length—they carry a big stick, biologically speaking. “We can say with confidence that over 60% of human protein-coding genes are conserved targets of miRNAs,” wrote David Bartel and colleagues in 2009. [1]

To date, some 16,772 miRNAs have been discovered and logged in miRBase, including 1,424 in humans. The question for researchers probing these molecules’ biology is, which miRNAs are active under a given set of experimental conditions, and how does that pattern change in the dynamic cellular environment?

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Accumulating evidence suggests that microRNAs (miRNAs) are important gene regulators, which can have critical roles in diverse biological processes including tumorigenesis. In this study, researchers from Nanjing University School of Medicine analyzed the miRNA expression profiles in non-small cell lung carcinoma (NSCLC) by use of a miRNA microarray platform and identified 40 differentially expressed miRNAs.

They showed that miRNA (miR)-451 was the most downregulated in NSCLC tissues. The expression level of miR-451 was found to be significantly correlated with tumor differentiation, pathological stage and lymph-node metastasis. Moreover, low miR-451 expression level was also correlated with shorter overall survival of NSCLC patients (P<0.001). Ectopic miR-451 expression significantly suppressed the in vitro proliferation and colony formation of NSCLC cells and the development of tumors in nude mice by enhancing apoptosis, which might be associated with inactivation of Akt signaling pathway. Interestingly, ectopic miR-451 expression could significantly inhibit RAB14 protein expression and decrease a luciferase-reporter activity containing the RAB14 3′-untranslated region (UTR). In addition, RNA interference silencing of RAB14 gene could recapitulate the tumor suppressor function of miR-451, whereas restoration of RAB14 expression could partially attenuate the tumor suppressor function of miR-451 in NSCLC cells. Furthermore, they also showed that strong positive immunoreactivity of RAB14 protein was significantly associated with downregulation of miR-451 (P=0.01).

These findings suggest that miR-451 regulates survival of NSCLC cells partially through the downregulation of RAB14. Therefore, targeting with the miR-451/RAB14 interaction might serve as a novel therapeutic application to treat NSCLC patients.

Wang R, Wang ZX, Yang JS, Pan X, De W, Chen LB. (2011) MicroRNA-451 functions as a tumor suppressor in human non-small cell lung cancer by targeting ras-related protein 14 (RAB14). Oncogene [Epub ahead of print]. [abstract]

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The Art of MicroRNA Research

by Chris on January 24, 2011

in Publications

The incredible enthusiasm for miRNAs as a novel class of functional regulators of tissue maintenance and stress responses demands for appropriate and reliable research tools. This review summarizes many of the currently available basic tools and describes the most widely used approaches for miRNA research to date. Although it will be the combination of several validated research methods that will enable researchers to validate the relevance or contribution of a miRNA to a certain phenotype, some caution should be taken to circumvent erroneous interpretation of data.

van Rooij E. (2011) The Art of MicroRNA Research. Circ Res 108(2), 219-34. [article]

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Accurate Molecular Classification of Kidney Cancer Subtypes Using MicroRNA Signature

January 21, 2011

Renal cell carcinoma (RCC) represents a spectrum of histologic subtypes that are morphologically and cytogenetically distinct. Distinguishing RCC subtypes is of clinical importance because they have different prognoses and subsequently different management plans. Attempts to distinguishing between the subtypes are usually made by morphologic assessment, which is often inconclusive and not always accurate. This can […]

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microRNAs a critical factor in cerebral, renal, and cardiovascular ischemia

January 11, 2011

MicroRNAs are approximately 22 nucleotide long, noncoding RNAs that extensively mediate post-transcriptional gene expression.  They control cellular function by either degrading mRNAs or arresting their translation. MicroRNAs are involved in various critical functions, including the regulation of cellular differentiation, proliferation, angiogenesis, and apoptosis as well as (patho)physiologic conditions such as tumor progression/regression and cholesterol/glucose homeostasis. […]

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Patterns of microRNA Expression in Non-Human Primate Cells Correlate with Neoplastic Development In Vitro

December 28, 2010

Neoplastic development represents cumulative genetic and epigenetic events leading to the emergence of cells that can attain a tumorigenic phenotype. Neoplastic transformation of cells cultured in vitro can be induced by several methods, such as treatment with chemical carcinogens or radiation, viral infection, or the introduction of oncogenes. To help understand how tumors originate and […]

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Normalization strategies for microRNA profiling experiments

November 24, 2010

Normalization strategies for microRNA profiling experiments: a ‘normal’ way to a hidden layer of complexity? MicroRNA (miRNA) profiling is a first important step in elucidating miRNA functions. Real time quantitative PCR (RT-qPCR) and microarray hybridization approaches as well as ultra high throughput sequencing of miRNAs (small RNA-seq) are popular and widely used profiling methods. All […]

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MicroRNA Regulation of Kinase Activity

March 10, 2010

It is known that Rho-associated kinase (ROCK) signaling plays a fundamental role in regulating cell morphology, adhesion, and motility and that aberrant expression of ROCK is related to tumor metastases and poor clinical outcome. Researchers at Tufts University proposed that ROCK may enhance the metastatic propensity of breast cancer cells by promoting the c-Myc pathway, […]

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