plasma

And a second webinar this week presented by abcam:

Time:

Thursday, June 16, 2016
3pm-4pm CET / 2pm-3pm GMT / 9am-10am EST

Webinar Overview:

Drug‑induced cardiac injury (DICI) detection remains a major safety issue in drug development. While circulating microRNAs (miRs) have emerged as promising translational biomarkers, novel early detection biomarkers of cardiotoxicity are needed to evaluate toxicity in a time-dependent manner.

This webinar will review: [click to continue…]

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New protocols improve detection of microRNAs for diagnosis

Philadelphia, PA, — MicroRNAs (miRNAs) that regulate processes including fertilization, development, and aging show promise as biomarkers of disease. They can be collected from routinely collected fluids such as blood, saliva, and urine. However, a number of factors can interfere with the accuracy of miRNA tests. In a study published online today in the Journal of Molecular Diagnostics, a group of researchers provide clear procedures for the collection and analysis of miRNA, significantly improving their diagnostic accuracy.

“Our study demonstrates that inherent differences in biological samples and the methods used to collect and analyze them can dramatically affect the detection and quantitation of microRNAs,” reports lead investigator Dominik M. Duelli, PhD, Department of Cellular and Molecular Pharmacology, Chicago Medical School at Rosalind Franklin University of Medicine and Science. “We developed measures to overcome the interfering activities and improved the sensitivity of miRNA detection up to 30-fold.”

Over 1,900 miRNAs exist in the human body. Deregulation of specific miRNAs is associated with disease. Measuring the amount of miRNAs in [click to continue…]

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microRNAs as Biomarkers in Plasma

by Chris on October 20, 2010

in Technical Article

MicroRNAs (miRNAs) are small non-coding RNAs found in eukaryotic organisms that regulate gene expression. Dismissed as “junk” until about a decade ago, it is now widely accepted that they play an important functional role in a wide array of cellular processes.

MiRNAs regulate gene expression by targeting individual or multiple messenger RNAs (mRNAs). Perfect or partial base pairing with the target mRNA promotes cleavage of the RNA in plants and inhibition of protein translation in animals. Many studies have demonstrated that dysregulation of these miRNAs is associated with various diseases suggesting there is potential for use of miRNAs in diagnosis and treatment.

Much of the study of miRNA and disease has focused on cancer and neurological disorders. Not surprising since cancer is the leading cause of death worldwide in 2010 and it is estimated that neurological disorders may affect as many as a billion people. There are urgent needs for early diagnosis and treatment in both areas.

Several miRNAs have been linked with various types of cancer due to their regulatory role in cellular development, including: differentiation, proliferation, and apoptosis. Neural miRNAs are known to be involved at various stages of synaptic development, including: dendritogenesis, synapse formation and synapse maturation. Since miRNAs exhibit specific expression profiles in tissues and tumor cells it makes sense that they could serve as biomarkers for these and other diseases.  (read more… )

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